Inhalation Facilities and Equipment

Inhalation Equipment at Vivotecnia Research:

Aerosol generator units

  • Test item preparation: Specialized equipment to disaggregate solid test items (Ball mill, mechanical press, sieve 2mm to 20µm)
  • Aerosol can be delivered at controlled temperature and relative humidity (0-80%) in order to balance artifacts due to dry air damage of respiratory tract organs and test item hydrolysis
  • Glass tubes for conducting the aerosol are inert to most chemicals, allowing work with corrosive or acidic test items as well as visual identification of deposits and condensations
  • Rotating brush generators (CR3020/PALAS RBG2000) and TOPAS Dust Dispenser SAG410 used for solid aerosol generation
  • Liquid aerosols nebulizer
  • 15 ISO based tobacco smoke aerosol generators
  • Vapour generator
  • Carbon nanotubes toxicology via dust generator (TOPAS SAG410)

Worldwide unique glass exposure tubes

…guarantee optimal thermal conducting characteristics, no adverse effects on testis due to temperature increase as observed in plastic tubes

…allow the sealing of exposure chambers individually:

  • for the performance of in situ pletismography and urianalysis while the animal is being exposed
  • protecting the personnel while allowing for rapid detection of aerosol deposits
  • no animal cross contamination

Exposure towers

  • 64 positions accommodate up to 56 rats and 8 sampling points
  • Nose only flow past principle as well as whole body
  • Chambers design avoids exhaled air inhaled by other animals
  • Steady state reached rapidly (lower chamber volume)
  • Minimal variation in aerosol concentration between levels

Facilities

  • Facilities endowed with 12 inhalation chambers for up to three studies in parallel
  • Negative control chambers in separate rooms, animals hosted in different rooms to avoid cross contaminations
  • Directed individual vertical ventilation control in direction of gravity:
    • reduces cross contamination
    • protects personnel while extracting animals
    • enhances thermal stability in the chambers

Atmosphere monitoring

  • Concentration analysis: Filter sampling and total particles matter by gravimetric measurement
  • On line monitoring: Casella Pro Dust
  • Particle size Distribution analysis: Cascade impactor
  • Impingers for quantification of gases and vapor atmospheres

Supporting services

  • Analytical and bioanalytical (HPLC)
  • In situ plethysmography