Mini Ames test
Vivotecnia offers mini AMES test as a pre-screening `miniaturized`version of the standard Salmonella reverse mutation assay (Ames test, OEDC 471). The mini Ames enables to perform a preliminary screning of the mutagenic potential of pharmaceuticals, agrochemicals, chemicals and cosmetics minimizing the usage of the test compound.
The mini Amest test offered by Vivotecnia uses the same bacterial strains, the same test principle and quality control specifically referred to in OECD Guideline 471, REACH and ICH guideline M7.
Mini Ames test is performed using two Salmonella strains: TA98 (frameshift mutation) and TA100 (base-pair substitutions). Both strains carry a defective (mutant) gene which prevents the bacterium from synthesizing the amino acid histidine unless mutations occur in certain genes. TA 98 and TA100 strains are the two most sensitive S. typhimurium strains for the detection of frame shift (TA98) and base-pair substitution (TA100). Other strains may be included upon request.
The assay is performed either in presence and absence of a metabolising system (eg, rat liver S9 fraction induced by Aroclor 1254). This approach enables to evaluate the mutagenic potential of the test compound as well as its metabolites.
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Vivotecnia offers the following studies for the analysis of genotoxicity: Ames Test, Comet Assay, Mini Ames Test